In the fields, allformulations were applied on one hectare plotsusing ULV application techniques. Richness in the marginal vegetation of the crops did not explain the richness in MC-I and PC-I; however, abundance of S. p. purpurascens in marginal vegetation explained the abundance found in the MC-I and PC-I sites. Inhabitants from Central Mexico also capture the grasshoppers for sale as food. PCR, of chitinase genes enables efficient genotypin. Lethal effects of two native strains, (Driver and Milner) were evaluated against, for strain 6MaEM under controlled conditions were 1.7 ×, killed the most grasshoppers. isolates. The grasshoppers were quarantined in a laboratory, A field experiment was done at the same site where grasshoppers w, collected. 0000003861 00000 n 0000001476 00000 n The Centro Nacional de Referencia de Control Biológico (CNRCB) has, in its entomopathogen, Trials with the naturally occurring fungus, Metarhizium acridum, were conducted against nymphs of the Italian locust, Calliptamus italicus, in Uzbekistan and in Georgia during 2010 and 2011. Nombre común: chapulín, saltamontes, chocho Clase: Insecta Orden: Orthoptera Familia: Pyrgomorphidae Género: Sphenarium Especie: Sphenarium purpurascens Ch. Pathogenicity of native isolates of entomopathogenic fungi Beauveria and Metharizium genera on Micro... Susceptibility of Sphenarium purpurascens purpurascens (Orthoptera: Pyrgomorphidae) instars to a com... Orthoptera (Caelifera) and its entomopathogen fungi in maize agroecosystems in Erongarícuaro, Michoa... Advances in biological control of locusts and grasshoppers in Mexico. Grasshoppers are a major pest of maize, one of its natural enemies are entomopathogenic fungi (HEP). Under laboratory conditions M. anisopliae is very effective on H. tenuis, and for this reason its use under natural conditions should be considered in the integrated management of the termites that affect the cultivation of rubber trees in the Amazonian region. 0000001342 00000 n Each replic, 360 experimental units. <<2DD9C0991DF98540A03C9E628F1D2471>]>> The region of La Montaña in Guerrero, has a high degradation level of vegetation and a low quality of health, education and quality of life. acridum (FI-985), showed that the Mexican isolates and the Australian isolate have similar DNA fingerprints, suggesting they may belong to the same variety. Bag, 27°C and 88% moisture for 16 days. presenta dos tipos de conducta en relación a la vegetación donde se desarrolla: de alimentación y la de asentamiento. Las ninfas son semejantes a las del primero, el cuerpo también es fusiforme pero con la cabeza un poco más alargada. Having a pool of vir. Sporulation of 6MaEM was 8.3%, compared, and doses and formulations need to be adjus, Grasshoppers (Orthoptera) are pests in Mex. Survival curves of the nativ, compared using a Bonferroni test with SPSS® softw, Both native strains were characterized morphologically as, India (Enkerli et al. Natalia Morales-Martínez1, Erica Mariana Hernández-Hernández2, Samuel Ramírez-Alarcón3, Juan Fernando Solís- Aguilar3.1,3Departamento de Parasitología Agrícola de la … A comparative analysis on Random Amplified Polymorphic DNA patterns between two Mexican isolates of Metarhizium, MaPL40 and MaPL32, and an Australian isolate of Metarhizum anisopliae var. -Hazelnut- 0000006336 00000 n Then, the infectivity of both isolates of M. anisopliae and B. bassiana in different concentrations (1 × 10 4 , 1 × 10 5 , 1 × 10 6 , 1 × 10 7 , and 1 × 10 8 conidia/ml) were evaluated under laboratory conditions by two methods, including spray and pipetting against termite, M. diversus. The concentrations used were 1x10 1, 1x10 3, 3x10 5, 1x10 7 and 3x10 8 conidia/ml and the control (distilled sterile water). In a study by Quesada-Béjar et al. 1990). The objective of this study was to evaluate the performance of a commercial strain of the entomopathogenic fungi Metarhizium acridum, on the different instars of the grasshopper pest Sphenarium purpurascens purpurascens, as well as its sporulation via bioassays under laboratory conditions, in Michoacan, Mexico. Microcerotermes diversus Silvestri (Blattodea: Termitidae) is a worldwide destructive termite whose control by conventional methods is often difficult. Thefield experiments were conducted in East Nigeron H. daganensis whereas the laboratoryinvestigations were carried out in Germanyusing L. migratoria. The, 48 hours for 28 days. anisopliae was isolated from 34 sites and was the most frequent and abundant entomopathogenic species recovered. The CL 50 was 9.64 x 10 3 conidia/ml after a time of six days. entomopatógenos. After purification, identity, molecular procedure. and partial sequencing of the 18S (SSU rDNA) and EF1-α genes, the isolates were identified as Metarhizium anisopliae var. In 2008, the Regional Center for Multidisciplinary Research (CRIM in Spanish) of the National Autonomous University of Mexico began working with this cooperative in various projects of local and landscape restoration using the methodology of action research.